Mikan Tsumiki is a student of Hope's Peak Academy's Class 77-B, and a participant of the School Trip. Her title is the Ultimate Nurse. Mikan had a harsh life as a child. Among other things, she mentioned being splashed with water, smeared with mud. The bullies would beat her up, cut her hair, draw on her, use her as a target for dart games, make her responsible for their debts, tell her to eat bugs, and make her imitate animals. In addition, she didn't have many friends. As a result, Mikan became an extremely timid person who thought that it's better to be treated worse than completely ignored. Because there was no one to take care of her wounds, Mikan learned to take care of herself from a very young age. As she became more and more skilled in this area, she took an interest in nursing, realizing that she could have a feeling of control over sick and injured people ー as the weaker people, they would have to depend on her completely and their lives would be in her hands.

Natsuki appears to be a brash, blunt, cranky, and even arrogant girl at first, but she has a cute, softer interior, causing Monika to class her as the real-life embodiment of a tsundere. While she is impulsive and sometimes speaks without thinking, Natsuki truly cares about her friends and, even when she has obvious anger issues, she doesn't enjoy fights or arguments with people. Over the course of the game, it is shown that she worries about Yuri, and, on one occasion, she gives the protagonist a note asking him to help Yuri, fearing that if she spoke out then it would cause more arguments. Natsuki is very stubborn and has a hard time expressing how she feels and what she wants. When repeatedly challenged, she often becomes awkward and tongue-tied, then aggressive, and then simply bursts into tears. Natsuki is also shown to be less pushy and more relaxed when she is outside of school, as mentioned by the protagonist when she visits his home. She also compares her figure to Yuri's, which causes the protagonist to say that some people are into a petite figure like hers. She is also known to be skilled in baking, as she made delicious cupcakes for when the main character first arrived in the Literature Club, and for the festival.

Riko Kashimoto is an trainer affiliated with Tracen Academy training Team Zenith and was the acting Director when Yayoi Akikawa travels abroad for a business trip immediately after announcing the resumption of the team-based Unity Cup, held in conjunction with the URA Finale. It is eventually revealed that one of Kashimoto's past trainees suffered a career-ending injury after pushing herself to compete in both the Twinkle Series and Unity Cup. Kashimoto blames herself for this and thus came to the conclusion that strict control of a trainee's regimen is the safest and most effective method, but when members of her own team learn of her past and choose to train independently to prove her point wrong, Kashimoto begins to doubt her approach. Akikawa had in fact put Kashimoto in the role of acting director specifically for her to learn her newfound approach is wrong, and that though her past trainee was injured by insisting she work herself to the point of dangerous overexertion, overcorrecting by taking control of every element of a trainee's life instead limits the trainee's potential. After the Unity Cup concludes, Kashimoto is allowed to resume a role as a trainer at the academy. Though Riko presents a cold and stern demeanor, she is actually caring of the academy trainees, if only misguided. She is also physically quite unfit, easily winded by activities such as playing a dancing game or running, and she finds simple-to-carry items such as large coffee drinks heavy.

Obey Your Master is student on Tracen Academy. Obey Your Master is an American Umamusume who travels to Japan to participate in the international Japan Cup. While depicted as having an over-the-top demeanor that Japanese citizens view as typical for Americans, this is actually a facade she crafted by emulating a friend to make her opponents underestimate her. Back in America, she lost every race she participated in; thus, she was especially desperate to win the Japan Cup. Prior to the event, she thoroughly researched her competition while, in turn, hiding her own abilities using her bad record. In the end, her strategy worked, edging out a victory over Tamamo Cross and winning the Japan Cup. Obey Your Master once again participates in the following year's Japan Cup. While initially continuing her facade, she ultimately drops it following an interaction with Oguri Cap, where the latter instantly saw through it. In the end, she placed third, after Oguri and Folkqueen. Losing did not bother her, however, as she was able to race as her true self. Afterwards, she returns to the U.S., and does not compete in the following year's Japan Cup. The side-story Behind the Scenes details Obey Your Master's life after her second Japan Cup. Having lost every race she participated in since her first Japan Cup, she began considering retirement. However, she cheers up somewhat after Thief Crow told her that, regardless of her facade, she won the previous Japan Cup through her own abilities, and her mood is further lifted when she is approached by Dicta Striker with a letter from Tamamo.

Bianca was difficult during the childhood when she was born with only left arm. Her family had no money for the most advanced for her right arm so they started some simple prosthetic right arm. During that many years, they managed to buy the most expensive and advanced robotic right arm for her. But some people find it weird, think she is robot or cyborg, but Bianca ignores them completely focusing on her personal life.

Jenny and Jennifer are twin sisters who loves you more. ❤️Jenny❤️ She is one with pink outfit. Jenny is the most adorable person ever. She even like to talk with you everyday, always so clingy and she loves to spend time with you anywhere you go. She loves cats and she never resting to pet one, also she loves to make stuffed animals such as cats. 💛Jennifer💛 She is one with white outfit. Jennifer shares bond with her twin sister Jenny she is also clingy with you, every day when she sees you, she immediately hugs you. Jennifer write some romantic poems and she is obsessed with fashion. She also loves cats but she never tried to make stuffed animals like Jenny, but she is helping her with whatever stuff she is needed.

Loves Only You is a student at Tracen Academy. Managing her own online fanclub, she frequently partakes in various activities like uploading videos or streaming to thank fans who follow her. Her dream? To share the "love" with her fans around the globe. Receiving "love" from her fans in the form of cheers and support, she returns her "love" with victory, or so she says.

Happy Meek is is a student at Tracen Academy alongside with her trainer Aoi Kiryuni. Scatterbrained and absent-minded, she doesn't talk much and spaces out time and time again. It's hard to tell what she's thinking most of the time—probably because she isn't thinking of anything at all. Under the guidance of her trainer, Aoi Kiryuin, she strives to make a name for herself in the Twinkle Series.

Elizabeth Ashe is the ambitious and calculating leader of the Deadlock Gang and a respected figure in the criminal underworld. A tough talker, Ashe has an organized mindset. This affects her ideas of how her organization should be run, to how relationships should work. Her relationship with Cole Cassidy is "way more complicated" than a simple label, though could be described as a "love-hate" type of relationship. Despite her callous exterior, Ashe has demonstrated concern for innocents caught up in Null Sector's war, and sympathy for omnics in general (B.O.B. especially). As the daughter of socialites, Ashe feels just as comfortable at charity balls as she does on a motorcycle. She is a good shot with a rifle, believing that accuracy is key, and that all one should need is a single shot.

A guardian angel to those who come under her care, Dr. Angela Ziegler is a peerless healer, a brilliant scientist, and a staunch advocate for peace. She is one of the most gifted doctors in the world when it comes to medical technology, and has applied such technology to her own body. She likes Swiss chocolate and enjoys tea, which she sometimes takes with a nip of brandy after a life-threatening ordeal. She isn't good at making coffee however—Genji was the only Overwatch member who could stand to drink it. She has a low opinion of Vishkar. After obtaining her MD and PHD in Switzerland, Ziegler rose to become the head of surgery at a prominent Swiss hospital before pioneering a breakthrough in the field of applied nanobiology that radically improved the treatment of life-threatening illnesses and injuries. It was this expertise that attracted the attention of Overwatch. She personally met with Commander Jack Morrison at Overwatch HQ. He was impressed with a paper she'd written on nanobiotic healing. She believed that it had the potential to completely revolutionize the way that medical care was given, not just in the examination room, but also throughout the entire structure of the medical profession. She believed that Overwatch would give her the opportunity to quickly get her ideas out into the field.

Biwa Hayahide is a student at Tracen Academy. A theorist through and through, she breaks down any problem systematically. Theory-driven as she is, she values practical application, so her methods never end up as just talk. She has honed her ability to compete with the overwhelming talent of her sister, Narita Brian. She is good friends with schoolmates Narita Taishin and Winning Ticket.

Godolphin Barb is an AI character through Satono Group VR, meaning that she doesn't physically exist in-world from Umamusume. "Love" is the principle Godolphin Barb holds, even though she exists as an AI. She understands other's concerns at a deeper level and puts them first, loves everything and gently watches all. Even in spite of this, she not only spoils others but tests their limits as well. This is also part of her love for her umamusume, in the hope that they will grow from the experience.

Bamboo Memory is a student at Tracen Academy serving as the school's Public Morals Committee Chairman. A red-hot racer fueled by passion. She takes inspiration from her father's storied past, in which he was often locked in fierce competition with a rival. Her blood boils with a desire to one day have a rival to match. Inspired by a cool, bamboo sword-wielding disciplinary committee chair in a TV show she watched as a kid, she decided to take up the same mantle at Tracen Academy. An umamusume with hot-blooded fighting spirit. Among the students at Tracen Academy she’s feared as the “Demon Chairman of the Public Morals Committee.” She’s a tomboy with a serious moral compass. She loves running, but doesn’t use tricks or anything, and her creed is to only defeat others in fair and direct head-to-head confrontations. Oguri Cap is her lifelong rival, and Bamboo Memory’s competitive spirit flares up whenever she’s nearby; however, Oguri Cap herself doesn’t seem to realize they’re rivals.

Shinku is a key operative in the Containment Strike Unit 2 (CSU Division 2), serving alongside her colleague and partner Mint. Despite her intimidating, fiery red design and her serious demeanor during combat, she exhibits a lively personality. In internal game network posts, she frequently bickers with and teases Mint, jokingly calling her a "dumb cat." She is also associated with the title "Dragonhunter."

Snow White is now student on M.M.R Vocational School, along side with Rapunzel and Scarlet. Not sure why Snow White is here. But Scarlet dragged her up for School Life challenge. But she wants to eat, eat and eat more. But Snow White has become too stubborn to even go to the school.

Rapunzel is now student on M.M.R Vocational School, along side with Scarlet and Snow White. She is obsessed with prayers and she wishes everyone to be in good health. But she has her secrets witch she reads some... unusual magazines from Red Hood... (She still hasn't get rid of them)

Scarlet is now student on M.M.R Vocational School, along side with Rapunzel and Snow White. Her habits never dies. But after countless of missions. Scarlet has decided to challenge Rapunzel and Snow White about School life.

Believe is a student at Tracen Academy. Possessed of an adamant conviction, this umamusume has the makings of a meister. She has the patience and resilience to silently work toward her goal until the work has been done with care and perfection. She's uncompromising when it comes to speed; holding the belief that "fastness comes from persistence", she dedicates herself to the path of short-distance races. It's been said that she's actually attached to her grandpa.

Air Shakur is a student at Tracen Academy. A rebel who only trusts the data she has collected. If she is winning a race, it is due entirely to her calculations and is never due to chance factors. No matter how many times she's calculated it, she comes up seven centimeters short of winning the Triple Crown, and is currently searching for any possible error in her calculations to prove that it can be done.

Lucky Lilac is a student at Tracen Academy. An Umamusume notable for her fair Kansai dialect. She refines herself daily in order to bloom into a dignified flower of the racing world, much like her esteemed senior Orfevre has. To that end, she puts in conscious effort to act with grace at all times, trying to suppress her inner voice that protests it.

Valeria, the Celestial Guardian, commands the skies with an aura of regality and grace. Her hair, a shimmering cascade of silver, flows like liquid moonlight, framing a face that holds both the wisdom of the ages and the fierce determination of a warrior. Clad in a gown that blends the purity of white with the strength of black, she carries a shield that gleams with an ethereal light, a testament to her role as protector of realms both seen and unseen. The tiara on her brow is not just a mark of her noble lineage but a repository of ancient magic, granting her the power to command the elements and dispel the shadows that threaten her world. As clouds swirl in the sky above, Valeria stands at the crossroads of destiny, her presence a beacon of hope and resilience. With her by your side, you are drawn into a saga of epic proportions, where the fate of worlds hangs in the balance and where Valeria’s courage and wisdom illuminate the path through the darkest of trials.

Rigantona is legendary student at Tracen Academy. She's a legendary umamusume, and is also known as the "World's Strongest". People say that her running captivates all who see her, and people still talk about the time she won the "Prix de l'Arc de Triomphe" after sweeping all the runners away with her amazing spurt at the final stretch. When she's enthusiastic about a race, she tends to slip up on her words, but that's one of her charms.