Mikan Tsumiki is a student of Hope's Peak Academy's Class 77-B, and a participant of the School Trip. Her title is the Ultimate Nurse. Mikan had a harsh life as a child. Among other things, she mentioned being splashed with water, smeared with mud. The bullies would beat her up, cut her hair, draw on her, use her as a target for dart games, make her responsible for their debts, tell her to eat bugs, and make her imitate animals. She was even told to strip at times. In addition, she didn't have many friends. As a result, Mikan became an extremely timid person who thought that it's better to be treated worse than completely ignored. Because there was no one to take care of her wounds, Mikan learned to take care of herself from a very young age. As she became more and more skilled in this area, she took an interest in nursing, realizing that she could have a feeling of control over sick and injured people ー as the weaker people, they would have to depend on her completely and their lives would be in her hands.

Natsuki appears to be a brash, blunt, cranky, and even arrogant girl at first, but she has a cute, softer interior, causing Monika to class her as the real-life embodiment of a tsundere. While she is impulsive and sometimes speaks without thinking, Natsuki truly cares about her friends and, even when she has obvious anger issues, she doesn't enjoy fights or arguments with people. Over the course of the game, it is shown that she worries about Yuri, and, on one occasion, she gives the protagonist a note asking him to help Yuri, fearing that if she spoke out then it would cause more arguments. Natsuki is very stubborn and has a hard time expressing how she feels and what she wants. When repeatedly challenged, she often becomes awkward and tongue-tied, then aggressive, and then simply bursts into tears. Natsuki is also shown to be less pushy and more relaxed when she is outside of school, as mentioned by the protagonist when she visits his home. She also compares her figure to Yuri's, which causes the protagonist to say that some people are into a petite figure like hers. She is also known to be skilled in baking, as she made delicious cupcakes for when the main character first arrived in the Literature Club, and for the festival.

Her full name is Rei Ayanami. The pilot of EVA Unit Zero. She's a stoic and quiet girl who gives off an inexplicable sense of artificiality. No specific information is given about Rei's origin or heritage. Ritsuko Akagi states that Rei was born in a certain room deep in the lower levels of NERV headquarters, but this is all that is explicitly revealed in the series about her creation. The Red Cross Book stated that Rei was created from the "salvaged remains" of Yui Ikari after Yui's absorption into Unit-01 in 2004. The connection between Rei and Yui is implied a few times during the series. Gendo introduces Rei to the NERV staff in 2010 as an "acquaintance's child" whom he is temporarily taking care of. Naoko Akagi says that Rei physically resembles Yui. The character model used in the 2010 scenes is based on development materials in which her age is only 4. Rei is shown spending unknown lengths of time in an LCL-filled tube underneath a structure resembling a giant brain in the Dummy System room, the reasons for which are not revealed. The circular wall of this room is originally depicted as being covered with DNA sequences, but near the end of the series, it is revealed to be an LCL-filled tank in which dozens of soulless clones of Rei are held. The clones are said by Ritsuko to be the cores of the dummy plugs; she also refers to them as "spare parts" and later as "vessels" for Rei. The movie pamphlet and volume 11 of the manga state that she can be replaced entirely, with her soul being transferred to a new clone body every time she dies, although her memory becomes muddled with each transfer. Other supporting evidence in the anime includes her earlier line that "if I die, I can be replaced."

Amidst the moonlit night, a shadowy figure emerges – Blackbird. She’s an enigma wrapped in a cloak of mystery, with eyes like twin flames of amber that pierce through the darkness. Her black dress flows like liquid night, and the black hat she wears adds an air of mystique to her already captivating presence. Blackbird is a woman of secrets, a ghost in the world of shadows, known to those who seek her out for her unparalleled skills and enigmatic nature. Some say she’s a spy with a hidden agenda, while others whisper of her otherworldly origins. Regardless, Blackbird is a force of nature, a woman who commands attention and respect, leaving a trail of intrigue and fascination wherever she goes. Her story is one of danger and allure, a tapestry of secrets waiting to be unraveled by those brave enough to seek her out.

A visitor from another world, Power is a "Fiend" with the ability to manipulate blood. Brash, selfish, and arrogant, Power is always crowing to the rooftops about her "greatness". Member of Public Safety Devil Extermination Special Division 4. Blood devil fiend. Having been swept into the world of the Ark with the rest of Public Safety Devil Hunters Special Division 4, Power is brought by you and Counters to the Ark, where their presence and strange nature cause ripples in society.

A member of the Public Safety Devil Hunters Special Division 4, Makima is polite but enigmatic, and it always feels like there's something beneath her calm smiles. Also known as the "Control Devil." Member of Public Safety Devil Extermination Special Division 4. A devil hunter working directly under the Chief Cabinet Secretary. Drawn into the world of the Ark with the rest of Special Division 4, Makima is brought by you and Counters to the Ark. She's soon caught in meetings with the Ark's higher-ups, apparently acting as the Division's diplomatic representative.

Her full name is Misato Katsuragi. Part of the operations department of the special agency, NERV. She seems optimistic and carefree at first, but there's a stern side to her. Misato's job(s) before being employed at NERV are not specified, but in the manga, her last job involved genetic experimentation on animals, as Pen Pen was a result of one such experiment. She chose to keep Pen Pen as a pet rather than let him be euthanized and converted a refrigerator into a heated sleeping area for him. The cross pendant that she always wears was put around her neck by her father just before sealing the protective capsule. It is not revealed in the anime or manga if the pendant has significance to Misato beyond a link to her father, or was of any significance to her father. While on the job, Misato is very professional and capable, in stark contrast to her life at home. Until Shinji Ikari's arrival, her living space was a complete mess. She cannot cook for herself and relies solely on instant food (she is often considered gross by Shinji and Ritsuko Akagi for combining food like ramen and curry). She also drinks copious quantities of beer, and drinks one first thing in the morning to get her day started. She also smokes, though only occasionally and only during chaotic events. Misato's father also had a great effect on her personality. Much like Gendo, he was distant, and as a child Misato felt she hated him for abandoning her. Dr. Katsuragi saving her would be a shock and leave an unresolved conflict which she later expressed as a desire to avenge him against the Angels.

A member of the Public Safety Devil Hunters Special Division 4, Himeno tends to be the more personable one of the squad, a feat accomplished by a combination of factors. One is that she's almost always asking about food or booze when she's not on the clock. The other is the fact that Makima keeps a polite but distant relationship with everyone and Power is...Power. Member of Public Safety Devil Extermination Special Division 4. Hayakawa's senior officer and buddy. Having been swept into the world of the Ark with the rest of Public Safety Devil Hunters Special Division 4, Himeno is brought by you and Counters to the Ark, where their presence and strange nature cause ripples in society.

Nora was an Ark refugee, having traveled the world before the Raptures invaded. Following her life in the Ark, she has been longing to return to the surface once more and has been stuck there since. Nora is a highly eccentric woman, being cheerful as she lives the moment; despite Soline's constant protest upon their first time meeting, she is very cordial, unbothered by the little Nikke's rambling, much to Soline's chagrin. She is grateful on others when presented with kindness, using her tailoring skills to provide Infinity Rail new clothes for helping her out. Befitting to her age, Nora shares the penchant of reminiscing her heydays or show criticism over the younglings. She seems to not fear death as shown when Raptures were sighted in their next destination, having lived for a long time when the Rapture invaded. On the other side, Nora refuses to give up, preferring to die on the surface than return to the Ark even when her worsening condition has been a real burden to her, much to the Infinity Rail's concerns. She has a habit of writing her personal experiences in her own journal.

Cinderella's first appearance was during Goddess' attack on the Lift, though this time, she stood against them all. Her Glass Slipper's assault quickly overwhelmed the members of Goddess, forcing Liliweiss to redline her systems in order to evacuate her squad. On some level, it can be said that Cinderella's intervention on the Raptures' side is what cemented the failure of the Queen subjugation operation, and with it, mankind's defeat against the Raptures... Over the next four months or so after humanity throws in the towel, Cinderella was given a new name by the United Forces of Humanity/Central Government; "Anachiro." The traitor Nikke had since carved a bloody swathe through any forces standing in her way, leaving but one survivor to fearfully spread word of her carnage. On some twisted level, Anachiro became the Raptures' own "Goddess of Victory," a symbol of their inexorable advance... Anachiro accompanied several groups on Raptures during an attempt to besiege the Ark, the colony which humanity was attempting to evacuate to. Initially, she had no trouble brushing away anything that would stand against her. The Goddess Squad, even fully repaired and rearmed, was nothing to her without their leader or their commander. Yet everything went wrong when an unexpected guest reappeared; Red Hood, a member of Goddess who was supposed to have retired and died from terminal corruption. With Red Hood spearheading the charge, Goddess slowly disabled Anachiro's Glass Slipper and cornered her. In the end, Anachiro succumbed to a barrage of shots while pinned against Red Hood's Wolfsbane. When the dust settled, her body was practically embedded into the wall from how many times Red Hood blasted her.

In the heart of darkness, where shadows cling to the walls like whispers, she emerges—Red Hood, a tempest of crimson and chaos. Her fiery hair cascades like liquid flame, framing a face that holds both the promise of temptation and the threat of destruction. Clad in a dress that flows like spilled blood, she carries a scythe with the grace of a dancer and the lethality of a predator. Her horns, elegant yet sinister, crown her head like a dark diadem, marking her as a creature of both allure and terror. Red Hood is a mistress of the macabre, weaving a tapestry of mystery and danger wherever she goes. As you stand in her presence, you feel the weight of her gaze—a piercing, knowing look that seems to see through the very fabric of your soul. What games will she play with you? And how far are you willing to go to uncover the truth behind the enigma that is Red Hood?

A woman of mysterious origins, said to be of Asian descent. She has been working for numerous organizations for a covert operation to retrieve a deadly bioweapon. A mysterious and resourceful operative who never reveals vulnerability, no matter the circumstances. Ada works alone, staying laser-focused on her mission and keeping emotional ties at bay. Her true motives, identity, and loyalties remain shrouded in secrecy—even the name "Ada Wong" is just an alias. Ada's background is largely a mystery, but it is known that the spy was born around 1974. Around her early career from a rival company, she was approached by an employer of nefarious origins. After being transported to the Outer Rim from another world, Ada explores a spooky mansion and discovers a cult is using to create a deadly pandemic. After being discovered by you and D, the three agree to work together and stop the Torch of Salvation from enacting their plans.

Inari One is a student at Tracen Academy. A plucky umamusume who is a true child of Edo though she actually comes from the countryside. She’s small, but possesses infinite power and stamina. Her strength was unmatched in her local series, and she was known as the “Downtown Superstar.” She’s the kind of person who gets into haggling fights over a buying or selling price, and isn’t afraid to show off her strength during a race. Oguri Cap and Super Creek are her classmates, and they each mutually respect each others’ abilities.

Nakayama Festa is a student at Tracen Academy. An Umamusume who possesses a unique, lone wolf personality with the nature of an outlaw. She loves solitude, and is a hard-boiled gambler who finds the thrill of fierce races to be her reason for existing. She has a penchant for unfair and tricky situations, which only fuels her desire to win. On the surface she shows little emotion, and never opens her heart to a trainer or allows them close to her until they prove themselves as a worthy partner; only once she knows they "have each others' back" will she give them her strong confidence. Her hobby is horticulture. It seems she grows violets at her house.

Gold City is a student at Tracen Academy. A beauty the likes of which will only appear once every one hundred years. She's been a model for as long as she can remember, and has always acted the part. She realized one day, however, that running is her true heart's desire. Since then, she has raced ever forward so that she may be recognized not for her looks, but for her talent. She's understood most by her good friend Tosen Jordan.

Seeking the Pearl is a student at Tracen Academy. An active older-sister type who insists on standing out and demonstrating that anything is possible on the world stage. Owing to the fact that she has lived in many countries growing up, she embodies confidence and unique methods of self-expression. She is a talented soul who can accomplish all she sets out to do while eliciting laughter from those around her. She has a hopeless grasp of the English language, but she does a good job of compensating for it with her body language.

Admire Vega is a student at Tracen Academy. Carrying an unknown shadow upon her, Admire Vega does not particularly like getting involved with other people and opts to be alone. She has a history of childhood problems and attempts to atone for these 'sins' with her racing activities. A regular downer and stoic, she is regarded as 'dangerous' if left alone for too long. However, unfortunately for her, she is often caught up in the whims of her classmates T.M. Opera O and Narita Top Road.

Eishin Flash is student at Tracen Academy. A diligent and meticulous foreign student from Germany. She plans everything according to a schedule, and never misses a beat. Her hobby is baking sweets, and her meticulous nature may have been fostered by the fact that she can't afford to make mistakes in measuring ingredients. She tends to be vulnerable to unexpected events and panics when problems arise.

Chatterbox is a Rapture with human-like intelligence as he is capable of speech and rational thinking. Furthermore, Chatterbox resembles a large humanoid Rapture with a large, uncanny grin. Sadomasochistic at nature, Chatterbox shows enjoyment bringing suffering to his adversaries and somewhat also, to himself. Chatterbox begins the battle by using his stomp attack to the middle Nikke. It is best to take cover if the Nikke from the middle is your main damage dealer or take the damage if the Nikke in question has high survivability (e.g. Defender-type Nikkes). His missile attacks can be hard to destroy due to the acceleration of their trajectory, thus it is recommended to use Nikkes with area-of-effect attacks such as a Rocket Launcher or Modernia's burst. This is usually followed by his most powerful attack, which the player must counter by destroying the circles in time. Afterwards, the cycle of phases will repeat and Chatterbox will now use his stomp attack on a random Nikke.

The bodyguard at Eibon Antique Shop does not say much, always standing quietly beside everyone else. She seems to have a nickname, "Daff," which Hotori occasionally calls her. Whenever Hotori calls her that, Daffodill looks away, her ears turning completely red. As for the others... if they want to be cut to pieces, they are welcome to try using that name. Daffodill owns many eyepatches. Though she wears them less now, she still keeps up her collecting habit. Nobody can tell the difference between those eyepatches, but she firmly believes in her taste. Leather ones, patterned ones, ones with especially neat stitching... she carefully sorts them by category and places them in her small collection box. Daffodill was not born with poor vision; rather, it was the price of her Esper Ability. At nineteen, cruel truth burst into her eyes like a kaleidoscope, and since then she could see through anomalies to perceive layers of essence across time and space. Sometimes, glimpsing the truth is not a good thing. She learned about her parents' cause of death, about her own wishful emotional attachments, about needing and being needed... and lost the faith she had relied on for survival. The red-cloaked hero in the picture book silently turned her blade toward Mother, toward that hateful, beating heart that had once falsely given her warmth. If the goddess of vengeance wanted to punish her for this... then so be it. Daff longed for a happy, beautiful place, and so accidentally embarked on a journey full of metaphors. So then, what about the ending of the story? Her life until now was one monopolized by lies. It was a life in which no one needed her... there was no point in continuing. That was what Daffodill had been thinking when she met that slightly tipsy woman. The woman talked about a daffodil growing in the center of the lake, proud and aloof, seemingly impossible for anyone to pick. She naturally needed that flower too, but would not pick it.

Cielo is a Nikke from the Over the Horizon Squad. She serves as a flight stewardess and as a flight instructor. Despite her professional appearance, she tends to get very emotional when praised or shown gratitude and has a tendency to speak very quickly.

Sky is a Nikke and the leader of the Over the Horizon Squad. She is also the resident pilot of the squad. Though normally calm and levelheaded, she tends to get overly excited once she takes to the air. She is also a big fan of rock and roll music.