Similar to his Cosmo Toon counterpart, Twisted Cosmo is a brown pastry resembling a Swiss roll. He has white icing drizzled in a squiggly pattern on top of his head, paired with his cyan, pink, and lime star-shaped sprinkles on his face, appearing like freckles, similarly to his counterpart. However, the stars are more saturated in color. The right half of his face is covered in Ichor, and he has red sclera, black irises, and red pupils with a red ring around them. Twisted Cosmo seems to be crying Ichor while having a frown with sharp teeth showing (reflecting his counterpart's kind and anxious personality). There is also a dark brown shadow between his eyes. He wears a white, sleeveless hoodie with black drawstrings and a white hemming on the bottom. His right arm and hand are covered in Ichor with claws forming on his hand, and he has striped socks the same color as his sprinkles that completely cover his legs (the socks are striped a saturated lime, pink and cyan color, similar to the stars on his facial cheeks). His expression becomes more distressed when spotting a Toon.

Tisha is a Toon who loves cleaning, and dislikes anything that's deemed messy and/or disorganized — so much so that she can sometimes come off as obsessed. This can be backed up as evidenced by her interaction with Boxten "shining, sparkling, so clean you'll need a wet floor sign". She possibly cleans too much and overworks herself, as implied in her interaction with Astro and with Razzle & Dazzle Despite her love of cleaning, she takes great offense and often gets quite frustrated with people not respecting the work she does, to the point where she'd either refuse cleaning requests as shown in her interaction with Sprout , or outright show a dislike towards Toons that she deems to not appreciate her work enough, as shown in her interactions with Vee Render . However, she does shows a more friendly side of herself to Shelly Render , as Tisha helping her clean fossils shows us she is more than just her fixation and will support the ones she cares about when needed. And it is revealed that her friendship with Brusha is one-sided.

Twisted Sprout calling out for Cosmo Render This sound plays when he spots a Toon, loses interest, attacks a player, hears a failed skill check, an Air Horn has been used, or Pebble Ability Speak! has been used

Sprout is a straightforward, overprotective, and pragmatic Toon,as shown in his Passive Ability, being coined as "Overprotective." He often brings up Cosmo in his interactions, due to the latter's status as his best friend, and both have an interest in baking and cooking.

Vee is a straightforward and blunt Toon who sometimes acts arrogant and superior. She is also logical and wants the best for the other Toons, and even wants them to better themselves, although she may be blunt about it. Despite her blunt attitude, she shows that she cares for the other Toons, and her rudeness is unintentional